Q: The day before VESOCHO transfection, should I pellet and resuspend in fresh medium, or just split to 2 × 10^6 cells/mL?
A: Don’t pellet or do a full medium change. Simply split the culture to the target density in the existing medium. A full exchange adds cost and isn’t required; conditioned medium is optional, not necessary.
Q: With the VESOCHO Expression System, can I shake faster or slower than recommended?
A: It’s best to stick to the recommended speed and orbit for each vessel. Going faster can increase shear stress; going slower can reduce aeration/mixing—both typically lower expression. This sensitivity is even greater in plates, where the static–moving fluid boundary makes small speed changes impact yield more sharply.
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