High-titer expression of cytokines, enzymes, antibody fragments, membrane proteins, and fusions in 2–8 hours—then scale to 100 L with the exact same settings. The time–titer curve stays the same at every scale, so there’s nothing to re-engineer. Enjoy reliable batches, frictionless tech transfer, and clean, comparable data that gets you to decisions faster.
Our cell-free protein expression kit delivers robust, soluble expression from small domains/VHHs to multi-kb enzymes and receptor fragments—so you can compare constructs side-by-side without re-optimizing culture conditions. Length-linked drops in yield are mitigated by optimized 5′-UTRs, balanced energy systems, and optional solubility aids (e.g., fusion tags, chaperone supplements, nanodiscs for membrane segments). Typical applications include domain mapping and truncation scans, fusion-tag evaluation, antigen and reagent prep, and rapid feasibility checks for difficult or toxic proteins.
Batch-to-Batch Consistency
Each lot is manufactured with tight QC on extract activity, nucleotide/energy components, salts, and cofactors, giving low run-to-run variability and reproducible kinetics across operators and plates. Matched buffers and standardized protocols make it easy to transfer methods between teams and sites. This consistency supports automation/HTP screening, assay qualification, comparability studies, and method bridging—so your rank-ordering of variants remains stable over time.
See detectable protein in ≤1 hour for rapid design–build–test cycles, and scale preparative reactions to achieve high titers (up to ~3 mg/mL) for screening or small-scale purification. The workflow runs from 10–100 µL microscale to mL-scale without retuning, enabling same-day evaluation of dozens of constructs and next-day downstream work (SDS-PAGE, LC-MS, activity assays). Ideal for hit triage, enzyme kinetics, diagnostic antigen generation, and pilot material ahead of cell-based production.